Part:BBa_K3956005
scl2 V Domain (globular)
The V Domain of scl2 collagen from S. pyogenes encodes a globular domain of collagen that stabilizes the protein in the absence of hydroxyproline.
The Scl2 V Globular domain is expressed in many bacterial forms of collagen. Although the function of the Scl2 V Globular domain is not apparent, it is hypothesized to play an important role in assembling the triple helix structure of the collagen.
Usage and Biology
Scl2, a streptococcal collagen-like protein, is a bacterial collagen that has been well-documented in recent years. The key distinction between bacterial and mammalian collagen is the hydroxyproline post-translational mechanisms: mammalian collagen requires this complex process whereas bacterial collagen does not. Past studies show that Scl2 has similar enough properties to human collagen to be used as biomaterials while also avoiding the lengthy processes of hydroxylation.
Going deeper into Scl2, most types of scl proteins, including Scl2, are naturally expressed in the human pathogen, Streptococcus pyogenes, also known as Group A streptococcal (GAS). The Scl2 proteins are virulence factors of S. pyogenes and contribute to the formation of biofilm adhesion and infection of different species (Squeglia et al 14). The Scl2 gene itself is made of several parts: the V domain, the triple helical CL domain, and the tail.
In CCA_San_Diego's project, the V domain was added before an enzyme cut site to separate it from the CL domain, and after a novl synthetic promoter and 5'UTR sequence.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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